Location: Home> Papers

Title: Hydrogen production of a salt tolerant strain Bacillus sp B2 from marine intertidal sludge
First author: Liu, HY (Liu, Hongyan)
To isolate a salt tolerant hydrogen-producing bacterium, we used the sludge from the intertidal zone of a bathing beach in Tianjin as inoculum to enrich hydrogen-producing bacteria. The sludge was treated by heat-shock pretreatment with three different temperature (80, 100 and 121A degrees C) respectively. A hydrogen-producing bacterium was isolated from the sludge pretreated at 80A degrees C by sandwich plate technique and identified using microscopic examination and 16S rDNA gene sequence analysis. The isolated bacterium was named as Bacillus sp. B2. The present study examined the hydrogen-producing ability of Bacillus sp. B2. The strain was able to produce hydrogen over a wide range of initial pH from 5.0 to 10.0, with an optimum at pH 7.0. The level of hydrogen production was also affected by the salt concentration. Strain B2 has unique capability to adapt high salt concentration. It could produce hydrogen at the salt concentration from 4 to 60aEuro degrees. The maximum of hydrogen-producing yield of strain B2 was 1.65 +/- A 0.04 mol H(2)/mol glucose (mean +/- A SE) at an initial pH value of 7.0 in marine culture conditions. Hydrogen production under fresh culture conditions reached a higher level than that in marine ones. As a result, it is likely that Bacillus sp. B2 could be applied to biohydrogen production using both marine and fresh organic waste.  
Contact the author:
Page number: 31-37
Issue: 1
Authors units:
PubYear: JAN 2012 
Volume: 28
Unit code: 133137
The full text link:
Full papers:
Departmens of first author:
Paper source:
Paper type:
Participation of the author: Wang, GC (Wang, Guangce)